Mobilized Peripheral Blood Leukopaks

A Mobilized Leukopak is a leukopak collected from a healthy, IRB-consented donor after stimulation with mobilizing agents such as G-CSF (Neupogen®) and/or Plerixafor (Mozobil®). These agents increase the number of circulating CD34+ hematopoietic stem and progenitor cells (HSPCs) in peripheral blood. As a result, mobilized leukopaks provide a significantly higher yield of stem and immune cells compared to standard leukopaks, making them ideal for cell and gene therapy research, CAR-T development, and regenerative medicine applications.

Standard leukopaks are collected from non-mobilized donors, providing a balanced mix of immune cells like T cells, B cells, NK cells, and monocytes.
Mobilized leukopaks, however, are enriched for CD34+ hematopoietic stem cells due to donor mobilization with G-CSF or Plerixafor. This enrichment allows for:

• Greater CD34+ cell recovery and consistency from a single donor

• Improved scalability of research and manufacturing processes

• Reduced donor-to-donor variability

CGT Global offers mobilized leukopaks collected using:

• G-CSF (Granulocyte Colony-Stimulating Factor, Neupogen®)

• Plerixafor (Mozobil®)

• G-CSF + Plerixafor combination protocols

Each mobilization method can be tailored to your study’s requirements. Combination mobilization generally provides the highest CD34+ enrichment and total nucleated cell (TNC) counts.

Mobilized leukopaks deliver a high concentration of CD34+ hematopoietic stem cells, which are essential for:

• Stem cell differentiation and expansion studies

• Gene editing and transduction research

• CAR-T and CAR-NK therapy development

• Hematopoietic reconstitution and transplant modeling

Researchers prefer mobilized products for consistent, high-yield CD34+ starting material that supports reproducible results in both preclinical and GMP-grade studies.

Typical Mobilized Leukopaks from CGT Global yield up to 20×10⁹ total nucleated cells (TNCs) per full bag, with CD34+ cell counts significantly higher than in non-mobilized collections. Actual yield may vary based on donor response, mobilization agent, and collection parameters.

Yes. CGT Global offers both Research-Use Only (RUO) and GMP-compliant Mobilized Leukopaks. GMP collections are performed under FDA-registered, CLIA-certified facilities with full documentation, chain of custody, and regulatory support for clinical manufacturing, IND-enabling studies, and early-phase trials.

Absolutely. CGT Global maintains an extensive donor registry and supports custom donor selection, including:

• Demographics: age, gender, ethnicity, BMI

• HLA, ABO, and RhD typing

• Medical history and lifestyle characteristics

• Additional screenings (e.g., CMV, Hepatitis, HIV, and other pathogens)

This customization enables precise donor matching and study reproducibility.

Our Mobilized Leukopaks consistently maintain ≥90% cell viability and are HBV, HCV, and HIV negative. All donors are IRB-approved and screened according to FDA and AABB standards. Each leukopak is processed using FDA-approved apheresis systems, ensuring maximum cell recovery and quality.

Yes. CGT Global provides:

• Fresh Mobilized Leukopaks — collected and shipped on the same day for time-sensitive applications.

• Cryopreserved Mobilized Leukopaks — processed immediately after collection to preserve high cell viability and recovery post-thaw.

Cryopreserved options are ideal for long-distance shipping or batch standardization across experiments.

Mobilized Leukopaks are shipped via FedEx®, UPS®, or World Courier®, with same-day delivery available in select U.S. cities near our facilities (Boston, Philadelphia, and Folsom). Cryopreserved shipments are packaged in validated cryoshippers to maintain cold chain integrity and viability during transit.

Yes. We offer extensive customization, including:

• Choice of mobilization agent (G-CSF, Plerixafor, or both)

• Custom buffers, media, or cryoprotectants

• Aliquoting and packaging by desired TNC or CD34+ cell count

• Closed-system processing for GMP studies

• Custom cryo-shipping configurations

For fresh leukopaks, collections are typically scheduled within days, with same-day processing and shipment. Cryopreserved mobilized leukopaks are available from inventory or can be prepared to order within 1–2 weeks, depending on customization.