Peripheral Blood Mononuclear Cells, Frozen

CGT Global Cryopreserved Human Cells in Cryovial
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Peripheral blood mononuclear cells are separated from peripheral blood by apheresis. PBMCs are screened for the expression CD45 and checked for viability using flow cytometry. This highly pure population of cells makes them ideal for further isolations of specific cell types within the PBMC fraction.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

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Since our founding in 2010, CGT Global has pursued our mission to transform healthcare as we accelerate cell and gene therapy research and clinical trials, streamline the commercialization of new treatments, and map the last mile to patient access to these life-changing remedies.  By innovating each stage in the cycle; development, commercialization, and delivery, we reduce the overall cost of the care and multiply access points so that millions can receive cutting edge, life-saving gene and cell therapies.

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Description

Peripheral blood mononuclear cells (PBMCs) are composed of lymphocytes (T cells, B cells, and NK cells), monocytes, dendritic cells, and stem/progenitor cells. These cells are critical components of the immune system and play key roles in the humoral and cell-mediated immune responses. PBMCs are commonly used in research and clinical applications in the areas of immunology, infectious disease, hematological malignancies, vaccine development, transplant therapy, and high-throughput screening.

 

 

Additional information

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Donor Attributes

Used in Research

Selected publications and protocols showing PBMC use in immunology and cell therapy workflows, including examples where PBMCs were purchased directly from StemExpress or isolated from StemExpress sourced starting material.


IVS of healthy donor PBMCs

Bio-protocol · 2025

Protocol describing in vitro stimulation workflows using healthy donor PBMCs in either fresh or cryopreserved format.

“HLA-A*02:01:01+ PBMCs were purchased from StemExpress…”

Direct StemExpress PBMC citation


CD47 and PD-L1 overexpression in proliferating human hepatocytes promotes immune tolerance

American Journal of Transplantation · 2022

Study using human PBMCs in a T cell assay to evaluate immune tolerance related responses.

“Human PBMCs were purchased from StemExpress…”

Direct StemExpress PBMC citation


Technical considerations and strategies for generating humanized immune system models

Computational and Structural Biotechnology Journal · 2024

PBMCs were used to develop and optimize humanized immune system models.

“Cryopreserved PBMCs were purchased from StemExpress…”

Direct StemExpress PBMC citation


PBMC-based immune response modulation in cancer research

Cancer Biology & Therapy · 2025

PBMCs were used to evaluate immune response modulation and therapeutic targeting strategies in cancer models.

PBMC · Immunotherapy


PBMC-driven immune signaling and functional analysis

Research Study (PDF)

PBMCs were used to analyze immune signaling pathways and cellular response mechanisms.

PBMC · Immune Signaling


Automated generation of immature dendritic cells

Journal of Immunological Methods · 2018

Whole blood purchased from StemExpress was processed to isolate PBMCs for dendritic cell generation.

“Whole blood… was purchased from StemExpress… PBMCs were extracted…”

PBMCs from whole blood


Dendritic cell mimicking scaffolds for T cell expansion

Materials Today Bio · 2022

PBMCs were enriched from leukopaks sourced from StemExpress for T cell expansion studies.

“Leukopak purchased from StemExpress… PBMCs were enriched…”

PBMCs from leukopak

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