Bone Marrow CD34+ Stem/Progenitor Cells, Fresh
CD34 is a glycosylated transmembrane protein and represents a well-established marker for human hematopoietic stem and progenitor cells in peripheral blood, bone marrow, and cord blood. CD34+ cells are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system through a process called hematopoiesis.
Since our founding in 2010, CGT Global has pursued our mission to transform healthcare as we accelerate cell and gene therapy research and clinical trials, streamline the commercialization of new treatments, and map the last mile to patient access to these life-changing remedies. By innovating each stage in the cycle; development, commercialization, and delivery, we reduce the overall cost of the care and multiply access points so that millions can receive cutting edge, life-saving gene and cell therapies.
Description
CD34 is a glycosylated transmembrane protein and represents a well-established marker for human hematopoietic stem and progenitor cells in peripheral blood, bone marrow, and cord blood. CD34+ cells are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system through a process called hematopoiesis. As hematopoietic stem cells progress through hematopoiesis they generate the myeloid (monocytes, macrophages, granulocytes, megakaryocytes, dendritic cells, erythrocytes) and lymphoid (T cells, B cells, NK cells) lineages. The highly specialized cells that arise from hematopoietic stem cells work collaboratively in defending the body against infection and disease.
Bone marrow mononuclear cells (MNCs) are separated from whole bone marrow by a density gradient centrifugation protocol.CD34+ cells are positively selected using immunomagnetic anti-CD34 (clone QBEND10) microbeads from bone marrow mononuclear cells. Isolated primary cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Additional information
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Product Information Sheet
Material Safety Data Sheet
Publications
- Eksioglu et al. (2017) Novel Therapeutic Approach to Improve Hematopoiesis in Low Risk MDS by Targeting MDSCs with the Fc-engineered CD33 Antibody BI 836858. Leukemia 1-9. doi:10.1038/leu.2017.21. Abstract
- Hudak et al. (2014) Glycocalyx Engineering Reveals a Siglec-Based Mechanism for NK Cell Immunoevasion. Nat Chem Biol 10: 69-75. doi:10.1038/nchembio.1388. Abstract
- Modarai et al. (2018) Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex. Mol Ther Nucl Acids 11: 116-129. doi.org/10.1016/j.omtn.2018.01.013. Article
- Cheng et al. (2019) S100A9-Induced Overexpression of PD-1/PD-L1 Contributes to Ineffective Hematopoiesis in Myelodysplastic Syndromes. Leukemia. doi.org/10.1038/s41375-019-0397-9. Article
- Lo et al. (2020) Potent In Vitro Activity of β-D-4ʹ-Chloromethyl-2ʹ-Deoxy-2ʹ-Fluorocytidine Against Nipah Virus. Antivir Res doi.org/10.1016/j.antiviral.2020.104712. Abstract
- Modarai et al. (2020) Precise and Error-Prone CRISPR-Directed Gene Editing Activity in Human CD34+ Cells Varies Widely Among Patient Samples. Gene Therapy. https://doi.org/10.1038/s41434-020-00192-z. Article
Figure 1. Representative flow analysis of CD34+ cells enriched from bone marrow mononuclear cells prior to cryopreservation.
Used in Research
Selected publications demonstrating the use of human bone marrow and bone marrow–derived cells sourced from StemExpress in regenerative medicine, stem cell, and immunology research.
Identification of osteogenic progenitor cell-targeted peptides that augment bone formation
Nature Communications · 2020
Bone marrow aspirates were used to study osteogenic progenitor cell targeting and bone formation mechanisms.
“Bone marrow aspirates were obtained from healthy human donors (StemExpress…)”
Bone Marrow · Regenerative Medicine
Subretinal versus intravitreal administration of human CD34+ bone marrow-derived stem cells
Annals of Translational Medicine · 2021
Bone marrow-derived CD34+ stem cells were used to evaluate regenerative therapies in retinal degeneration models.
“Fresh bone marrow from a healthy human donor was obtained from StemExpress…”
CD34+ Cells · Stem Cell Therapy
Effects of intravitreal injection of human CD34+ bone marrow stem cells
Experimental Eye Research · 2020
Bone marrow-derived CD34+ stem cells were used to investigate therapeutic effects in retinal disease models.
Stem Cells · Ophthalmology
Human mesenchymal stem cells suppress proliferation of activated lymphocytes
Cellular Immunology · 2016
Bone marrow-derived mesenchymal stem cells were used to evaluate immune suppression and regulatory T cell responses.
“Bone marrow-derived MSCs… were purchased from StemExpress (Placerville, CA)”
MSC · Immunology
Proteomic analysis of exosomes secreted from mesenchymal stromal cells
Biology · 2021
Bone marrow-derived mesenchymal stromal cells were used to study exosome secretion and cellular communication.
“Bone marrow specimens… purchased from StemExpress…”
MSC · Exosomes
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