Peripheral Blood CD4+CD45RA+ Naïve Helper T Cells, Frozen
Peripheral Blood CD4+CD45RA+ Naïve Helper T Cells, Frozen
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CGT Global is a Biospecimen Provider for a wide range of clinical and preclinical research projects. Our state-of-the-art laboratories and collection centers are located nationwide and provide end-to-end services: These services include:
- Process, isolate, and cryopreserve cells immediately after donor collection
- Amazing customer service and fast shipping
- Customize products to distinct study specifications
- Retain specific donor demographics and a safe, highly recallable donor pool
- HLA Typed Primary Cells
- Biobanked cells
Description
Naïve helper T cells are mature T cells that have left the thymus and have yet to be exposed to an antigen presented by MHC class II molecules of an antigen-presenting cell (APC). In humans, CD4+ naïve helper T cells are constantly recirculating into the secondary lymphoid tissues in order to optimize the opportunity of finding their specific antigen.
Human peripheral blood CD4+CD45RA+ naïve helper T cells are enriched by depleting cells expressing CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, CD45RO, TCRγ/δ, anti-HLA-DR, and CD235a from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+CD45RA+ naïve helper T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Additional information
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Product Information Sheet
Material Safety Data Sheet
Protocols
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Figure 1. Representative flow data of the enriched CD4+CD45RA+ cells (91.0%) from peripheral blood prior to cryopreservation.
