Bone Marrow Mesenchymal Stem Cells, Frozen

Bone Marrow Mesenchymal Stem Cells, Frozen

Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into the cells of the connective tissue in vitro, such as chondrocytes (cartilage), osteoblasts (bone) and adipocytes (fat). Although little is known of the phenotypic characteristics of MSCs in vivo, MSCs in culture are defined by the expression of CD73, CD90, and CD105 and the lack of CD11b, CD19, CD34, CD45, and HLA-DR. Cultured MSCs have shown promising applications in cell-based therapeutic strategies for specific tissues and cancer research

The CGT Global Difference 

CGT Global is a Biospecimen Provider for a wide range of clinical and preclinical research projects. Our state-of-the-art laboratories and collection centers are located nationwide and provide end-to-end services: These services include:   

  • Process, isolate, and cryopreserve cells immediately after donor collection 
  • Amazing customer service and fast shipping
  • Customize products to distinct study specifications 
  • Retain specific donor demographics and a safe, highly recallable donor pool 
  • HLA Typed Primary Cells
  • Biobanked cells
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Description

Bone marrow mononuclear cells (MNCs) are separated from whole bone marrow by a density gradient centrifugation protocol. MNCs are cultured for 9-14 days in a medium that promotes MSCs. After culture, adherent cells are checked for >90% expression of CD73, CD90, and CD105 and <5% expression of CD14, CD34, and CD45 by flow cytometry.

Please request for cells to be cultured in animal component free (ACF) and serum-free medium.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Additional information

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Anticoagulant

Format

Grade

Species

Cell and Tissue Source

Disease State

Donor Attributes

Protocols

Thawing Protocol

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