Peripheral Blood CD4+CD45RO+ Memory T Cells, Frozen

Human CD4+CD45RO+ memory T cells are isolated from peripheral blood mononuclear cells by depleting cells expressing CD8, CD14, CD16, CD19, CD36, CD45RA, CD56, CD123, TCRγ/δ, and CD235a using immunomagnetic particles leaving purified, untouched CD4+CD45RO+ memory T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Since our founding in 2010, CGT Global has pursued our mission to transform healthcare as we accelerate cell and gene therapy research and clinical trials, streamline the commercialization of new treatments, and map the last mile to patient access to these life-changing remedies.  By innovating each stage in the cycle; development, commercialization, and delivery, we reduce the overall cost of the care and multiply access points so that millions can receive cutting edge, life-saving gene and cell therapies.

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Description

Peripheral blood CD4+CD45RO+ memory T cells are formed after a naïve T cell has come into contact with an antigen. Memory T cells remain inactive until they encounter the same antigen and reactivate to form effector T cells. Having encountered the antigen previously this second encounter produces a faster and stronger immune response to eliminate the foreign invader or cancer cell.

 

Additional information

Anticoagulant

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Figure 1. Representative flow data of enriched CD4+CD45RO+ memory T cells from peripheral blood prior to cryopreservation.