Frozen Leukopaks
Frozen Leukopaks
Frozen Leukopaks are and collected from IRB-approved consented healthy donors at FDA registered collection clinics by qualified staff and cryopreserved on the same day.
The CGT Global Difference
CGT Global is a Biospecimen Provider for a wide range of clinical and preclinical research projects. Our state-of-the-art laboratories and collection centers are located nationwide and provide end-to-end services: These services include:
- Process, isolate, and cryopreserve cells immediately after donor collection
- Amazing customer service and fast shipping
- Customize products to distinct study specifications
- Retain specific donor demographics and a safe, highly recallable donor pool
- HLA Typed Primary Cells
- Biobanked cells
Description
CGT Global Frozen Leukopaks are collected from IRB consented healthy donors at our FDA-registered Stem Cell Collection Center. Cell-rich peripheral blood mononuclear cells (PBMCs) are extracted using a continuous flow centrifugation system. Leukopaks are collected directly into a sterile collection bag containing the anticoagulant ACD-A. Besides the PBMCs, which consist of lymphocytes and monocytes, each Leukopak will also contain minimal amounts of granulocytes and red blood cells.
The following are mean percentages of cell populations for a single donor Leukopak prior to cryopreservation:
- T cells – 55%
- Monocytes – 27%
- B cells – 9%
- NK cells – 8%
- CD34+ stem cells – 0.1%
- Granulocytes – 2.4%
We do not guarantee the percentage of cell populations in a Leukopak, as they can vary from donor to donor.
Leukopaks are quickly frozen after collection in a medium containing the cryoprotective agent CryoStorTM (10% DMSO) using a controlled rate freezer to ensure maximum viability.
Before cryopreservation, the Total nucleated cell (TNC) counts and viability testing are performed. For optimal cell recovery and viability, we recommend following our Thawing Protocol found under the Technical Resources tab. Deviations from this protocol can compromise viability and cell recovery.
For global shipments, we highly recommend shipping Frozen Leukopaks in an LN2 cryoshipper to maintain proper temperatures for the duration of the transit time. Upon arrival, Frozen Leukopaks should be stored in the vapor phase of liquid nitrogen or thawed immediately for use.
Custom Orders
Design the right Leukopak for your research needs. View our custom orders to see what options we have available.
Additional information
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Product One-Sheet
Product Information Sheet
Human Peripheral Blood Leukopak, Frozen – Product Information Sheet
Certificate of Analysis
Human Peripheral Blood Leukopak, Frozen – Sample CoA
Material Safety Data Sheet
Human Peripheral Blood Leukopak, Frozen – MSDS
