The CGT Global Difference
CGT Global is a Biospecimen Provider for a wide range of clinical and preclinical research projects. Our state-of-the-art laboratories and collection centers are located nationwide and provide end-to-end services: These services include:
- Process, isolate, and cryopreserve cells immediately after donor collection
- Amazing customer service and fast shipping
- Customize products to distinct study specifications
- Retain specific donor demographics and a safe, highly recallable donor pool
- HLA Typed Primary Cells
- Biobanked cells
Description
Peripheral blood CD4+CD45RO+ memory T cells are formed after a naïve T cell has come into contact with an antigen. Memory T cells remain inactive until they encounter the same antigen and reactivate to form effector T cells. Having encountered the antigen previously this second encounter produces a faster and stronger immune response to eliminate the foreign invader or cancer cell.
Human CD4+CD45RO+ memory T cells are isolated from peripheral blood mononuclear cells by depleting cells expressing CD8, CD14, CD16, CD19, CD36, CD45RA, CD56, CD123, TCRγ/δ, and CD235a using immunomagnetic particles leaving purified, untouched CD4+CD45RO+ memory T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Additional information
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Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
Protocols
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Figure 1. Representative flow data of enriched CD4+CD45RO+ memory T cells from peripheral blood prior to cryopreservation.