Peripheral Blood CD19+CD27- Naïve B Cells, Frozen
Peripheral blood CD19+CD27- naïve B cells are B cells that have not encountered an antigen. Upon antigen contact naïve B cells will undergo isotype class-switch recombination and somatic hypermutation and develop into plasma and memory B cells.
Human CD19+CD27– naïve B cells are isolated from peripheral blood mononuclear cells by means of negative selection. Cells expressing CD2, CD14, CD16, CD27, CD36, CD43, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD19+CD27– naïve B cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
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Figure 1. Representative flow data of peripheral blood enriched CD19+CD27– naive B cells prior to cryopreservation.